	   SnapGene      #lGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggGaGGCGGAGGTAGTGGAGGAGGGGGAAGCggaggcggagggagtGAATTCatggataccgacttatatgatgagtttgggaattatattggaccagagcttgattctgatgaagatgatgatgaattgggtagagagaccaaagatcttgatgagatggatgatgatgacgacgacgatgacgtaggagatcatgacgatgaccaccctgggatggaggtggtgctgcatgaggacaagaagtactacccaacagccgaggaggtgtatggtcctgaggtggagaccatagttcaagaggaagacactcagcctctcacagaacccattattaagccagtgaaaaccaagaaattcactctgatggagcagacattacctgttacggtgtatgagatggatttcttggcggatctgatggataactcagagctcatcagaaatgtgaccctttgtggacatctccaccatggcaagacatgttttgtggattgtttaattgaacagactcacccggaaatcagaaagcgctatgaccaagatctgtgctatactgacatcctcttcacagagcaagagagaggtgtaggcatcaaaagcactcctgtgacagtggtcttgccagacaccaaaggaaaatcttatctcttcaatatcatggacactccaggacatgtgaatttctctgatgaggtcacagctggcttgcgcatctcagatggagtggtccttttcattgatgctgctgagggggtgatgctgaacacagagcggctgatcaagcatgcggtgcaggagaggctggcagtcactgtgtgcatcaacaagattgaccggctgatcctggagctgaagctgcctccaactgatgcttattacaagctgcgccacattgtggatgaggtcaatggattaataagcatgtattccactgatgagaacctgatcctttccccactcctgggtaacgtctgcttctccagctcccagtacagcatctgcttcacgctgggctcctttgccaagatctatgccgacacctttggtgacattaattaccaagaatttgctaaaagactctggggtgacatctacttcaaccctaagacgcgaaagttcaccaaaaaggccccaactagcagctcccagagaagtttcgtggagtttatcttggagcctctttataagatcctcgcccaggttgtaggtgacgtggacaccagcctcccacggaccctagacgagcttggcatccacctgacgaaggaggagctgaagctgaacatccgccccttgctcaggctggtctgcaaaaagttctttggcgagttcacaggctttgtggacatgtgtgtgcagcatatcccttctccaaaggtgggcgccaagcccaagattgagcacacctacaccggtggtgtggactccgacctcggcgaggctatgagtgactgtgaccctgatggccccctgatgtgccacactactaagatgtacagcacagatgatggagtccagtttcacgcctttggccgggtgctgagtggcaccattcatgctgggcagcctgtgaaggtactgggggagaactacaccctggaggatgaggaagactcccagatatgcaccgtgggccgcctttggatctctgtggccaggtaccacatcgaggtgaaccgtgttcctgctggcaactgggttctgattgaaggtgttgatcaaccaattgtgaagacagcaaccataaccgaaccccgaggcaatgaggaggctcagattttccgacccttgaagttcaataccacatctgttatcaagattgctgtggagccagtcaacccctcagagctgcccaagatgcttgatggcctgcgcaaggtcaacaagagctatccatccctcaccaccaaggtggaggagtctggcgagcatgtgatcctgggcactggggagctctacctggactgtgtgatgcatgatttgcggaagatgtactcagagatagacatcaaggtggctgacccagttgtcacgttttgtgagacggtggtggaaacatcctccctcaagtgctttgctgaaacgcctaataagaagaacaagatcaccatgattgctgagcctcttgagaagggcctggcagaggacatagagaatgaggtggtccagattacgtggaacaggaagaagctgggagagttcttccagaccaagtacgattgggatctgctggctgcccgttccatctgggcttttggccctgatgcgactggccccaacattctggtggatgatactctgccctctgaggtggacaaggctcttcttggttcagtgaaggacagcatcgttcaaggtttccagtggggaaccagggagggccccctctgtgatgaattgattcggaatgtcaagtttaagatcctggatgcggtggttgcccaggagcccctgcaccggggcgggggccagatcatccccacagccaggagagtcgtctactctgccttcctcatggctactcctcgtctgatggagccttactactttgtagaggtccaggcccctgcagattgcgtctctgcagtttataccgtcctggccaggcgcagggggcacgtgactcaggatgcacccatcccaggctcccctctgtacaccatcaaagcttttatcccggccatcgactcttttggctttgagactgatctccggactcacacccagggacaagccttttctctgtctgtcttccaccactggcagattgtgcctggtgatcccctggacaagagcattgtcatccgccccttggagccacagccagctcctcacctggcccgggaattcatgatcaaaacccgccgtaggaagggcctcagtgaagatgtgagcatcagcaaattcttcgatgatcctatgttgctggaacttgccaaacaggatgttgtgctcaattaccccatgtgaGGATCCtGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGT  LÙ   *   )      )   6   )   ,   "   9            2   )   8   *   ;          1   6            :      $   3   (   $       *   8   +      #                         -               $          (   0   %   -      G      ,   4   &   0   $   9   $   0   "   .   $   $   (            *      !   5   2      *   2   "   ,   %   8                                  -   5      $               *               /   .   *                   %   #      !         )      .      ,   +   ?   (   ;   .   (   <   2   1               $   2   2                                    %               &   &       '         !      '      /   (      %   +   )               %   2   %   (   6      *   #   !   (   *   &             +   #         (                     &   9   +   *   "   8   =   %      &   -         <              /   B   >   '   2               &      /   )      1   )   0   "          6   <   )   *   /   ;      %       $   8   /   8        * ñ y‡KL#	$	o
bcÑsÐÕÖij§¨©®³ÂÃäÀÁê’U’2Ÿ!Ò" #) yzœ¸úN5M]„dÒÆãt|ÜPÄÍå‘3qvŠ÷r "÷Çf Ñ!ž"R# – ò �ˆ¶%	ø	p
4Öª¯FÎ í˜è“3Óx"æ"!#) · `ætËÑÄå(
™N•ÊŠþ€Ñ×koú´ïÂ³ëG†“V/ãµgË' "¦"6 C “�ûOz…ô6<EHTZ^ð:O£	P	ƒ	¾
Ê
'*etX¯²ÓÇ}Âî3Å<
4œ"¡ÈS#) N…a9¯}ö9¶¼ušQk¥V}ÎdÜó;r(¾ês-RØ Uã# g  "‡"Ÿ"¯", f{kÔ	ÄÐÙ¶	Ã	w ä&°y´ÐÈæ’I[a|˜$=ŽÑ#Þ²øÒ!"ù"M#" z¹¡š÷o&�þÉÓô
uÃß–>YÏÝ¹R#wFñý‹øº9 �D÷k‰Œ¸ÐQ=L¸|&	J	J
q
ì
–»¾5¼ä¬36¥#;¶Z—«°ìGLî6™»é4©Oø¬ ˆ!y" $žåê?·ù	\¯IóB˜²×í4a‚¿  — Lmå
×Ni¹OÏ'ªˆó”ßM’©ˆÔ 	!H!ç""# F ó þ˜ùDœê	¼	N‹¨1{/ÞÞUÔÿ[8 !2 œ açîu¥ÆÌÒ2Å 9WÍašÐ¹Òcæ5CìH‡Öy‰¡î%(Ó¦¶ìk¬Ë(õä °!Æ!"§") o ®õR©á¡æûIs!‚Y	?OÏË�lÿµÜäS´)”Êpz¤ N!‹"8 t 40\zô:	U	y	)
JmÕíÿD
‰¼ðø§ÃÿGÂ¡©ÄÝ1:|ÈÙìWrä	0äÿæyhÌ Ä * ¸ +ì±6¦�
î0–ª€‹�Ø7^¬Kpéû¢ßå�>Åà”BM_Ø( 3!; D üxÉÏ¶Âõm7=FIU[8;J_zè!	S	º	(Óu{º°”£9~/Îø{ÆÿÝã_Ÿ'Œ¤§ëxÉ  Pè»½ÏñP¿vxQ	Þ	²
f.…�ÈïÂU�·¾¢ñ¡!/"T# M ”ž_~	¿
dÛ;³Êññ’=|#>§\Qo ç ‹!·!1 X n{œ†ÝÆØ*–¤ãp6	„	Ë
+¬Yk”¼Ô|Ã4�µõ5M†ÍBï:Šç, |"õ"6 ˆ ïUw»âBêO¥%b‰	´
$0Y~áø4÷c‡½ÀVlyWÏW lsŠ)JÙ!	&E»4$ 3 h )!ˆ"Ù" 8‹”í:A°�
:U~ô<¹¿™Ûót.¯Vªè""°"#6# ‹ c†±Û\
Ÿ�›RÏÝ²+ë�£! "¸"#  ÓET“w+/	à	6h¿f·v¦	ese2St)äé!:  ° GÕ{¢¨«®ÌÑÕØÛçü
 ÁÖå		)	™	·	Û	ëöxv}èú'�±z|”µç\yÍÌ%>¤w!;" ½ glÚ‰*× $Ä¹ÂéÓ!ú"$ Ï |œÅq	Ä	¨Ý%Oïu®ß•AÉÕûˆ“œºo}ÒXÀÆßŸù 3 ]ñÆóÑR>	}	æ	7
Ù
¡êÄlåªÝÈZ—ÑJOb™ç8A�k³¾Æ ‰Þ ð ! "1"U"N#V#^#( Q]†Ùâ“Ý¢¨›žøp£'Þáäêùˆ
ï
!0$6$D`G†yªù‡šÙD#J#$ } É È:¸ e©ÿa–¦ÊÔA
M
t
õ
vû
—?ZÞºÒW½þ¶·`ª"  Ö {rºC_ìr0i
Äß�às³ÄÐSÆ¯xÚŒÐ»¼©„!* y 8CRé"+M¬…ß‘¿ÿRôLu	Êå-ÕP^ìüšò4ž¢Áõ†ó³!8 ‘døŠCMüš		´	¼
ò
s—¼Á=“¨V­47¦´$<q·ä¬±íJMPÚñ9š8¼Þì�ª&á6 ª + El ¹ÑR	a‰ò”Ù'	K
r
í
ÝãW½öÂæ,T‹qwÌ\˜ñ@8v‚!‰!z" < > »ñÀ>¹}c	{}¿Äåä˜Háãï7Öêï'Pl´­ :!¨!# R �5:y¬WK	e	ù6K»E[ÛZ�@5~änùßý ô!I";# O ë@¸íé
,h”0?Ròbƒ¶u  å %mŸæÊ«ú	Ö)	X™³ØSq ,!s!>" + `–½-@»Ñ	Z;½J§·°ôCî.5IÀ ª!	# 	 ³ ²Ë#Vó/(
]°ýÛ”�! @ Æ æ
çºŸ¸%39cà�We^¢` ’ • ú "!0!¹!,"µ" " ˜ Û ³GP«éNùLòd€ é 
!<!I!è"  Ÿ Mn ¤€#·
Á
#'j¤P 6(fð:“èªnÎ‰‘7‡ » r"- û d€¹ò¾Y‘
ZfÆØ7ÌõOc ÐEZ«‰ØôKj~•%âðKY}—x Õ "7"## G Ç™ó5•¾o{ßV±¸ G# ô T‰Ü3–ë]	½	_Œ"Ÿ%š±å+sáýf¡ß£ ¯ %!g!™!É! …ÿ+�ÿ	OµöGîòóŽ|0³¢Õ\ŽU î" Âj€ÏúE	©2Yi?ø@€—©9 !$ Jkè:RXÎ8	H	ô´Óçê3AD/oÔ×ï&ˆ�§êñö†!®!÷"  V ® v¦ÇÍÓÆ¡ï‹
bÑºÍˆŠ¢)¨í­é‡ïå ±!"¨" � Ä bï¾3wEZIÔU®clÌ$Ç!p"( T ¤ aˆ±íEùª	›…d)6?Uowåí-R\z|`Z›·Øæ)�;¥. ‹ Ü!(#0 ¯ S	7X?t{�¦M	Z	¨
�P‘>Mf ¶ÙWPTÚ…ç *T•]A*ËÎä™œ§0 7!O!æ!% Z p àpâ¢üÚrSÐÿ‡‰¾/^w&ÏZ<Gq•ä > � ÿ ~"Œ"–"-  „ öØçJ"ƒûD	g	Í	
„¾ë^ ƒ„‰Ý8cgkÝO~b`w… ‡Œ¥ W!]"Ü"   ªú[Š	ÈŒ®@ŽÌËm}ßå�µp{R!K"*#G J ¦ 5Y}žæß®±ÿ,ñ		,	;	V	z	†	Œ	§	%
-3K8KÌSmRyÃêî¢
á·ÃÅÞ;W}ÉÚå1 å„Õçh<diŠBá)" e 9]E{GYb
Ö–b÷¢ª2€íö
ÉDûk ž , 8 u ‘ © )1<˜õœ	

nyM ¡½ù†¨Ý 7”ã«É!8BåDÏgÁþ+mÍÅ z!4  1 ~å
*
«
E ÖEŠ@½íñ}¶Ä H-È\®XsR \¹ÉÚúz�Üè6]=eÜú ´ Ï !¼!ö!&  B³7‚5>
1—eê¤™àæ‘ÐÓ�´,b¯ÆáOÏü`û  ) c 4!“"0 ' ¹ rÆc²Øn#b…¢¬	2
�û«[Ùê_­6<¬(,]k›?^n¶€•CNÄÑH | ˜ l!Þ!=#$ 0ñm�	Í
ü
*Sš�Z~Œÿã	qG£éï§gqÒ.7�¥J 9 ,<J¦ÏíýÕ�
s§´Óž
à
>¢ïpñ2‚½Å8,Lü:+qy/…‘š¦°»ïô‚­,c‚ÙÂ!$ • xMsÊÐÃä4J\"	‰{hÛnùÁÍÿ�ìéuT öÊ& �"¥"å"#0 y„™óönGï9`®ÒO	&)v|±•»XÁíxj³Ï|¸Ç	Z`"—<�ü,Q¹Ý±"†"" E ý·P><K{é»	ä
ë
•[®»ãŠ«¤:h0Y–5‡Þ¨ATì. n s › íy8V�T	œ
IlÔC6bˆÛ þ
FRÞä (qx¥AL^xÊôy£ ã Š"$ •º¼^âouwy5	R	±
j““œÉð.÷ÜTžLÌ£¦æ [n $ Š AD’é$v“ì@ß	[
³
gbe†ž¿Vÿ¸1%(©º2 (!¢!è!#( ¼ \§ÅÐòÀQ	p	6
Ø
§ˆ$Nuùk­©Ü@{x¿Ãž½¸ v!0"U# Q«Þ¾Qd¨/¥h
‡
€ú	/ÔÃÑ#V¼¶ò Q º9B`ˆ™	Ü<JSpvãÚOŒ&?àuÞ5 9!§!ó!:#  * N ä l•±Ê"U'
Ú“Y¼-HRp t +!Œ!* ! Ú ú Ÿ¸ñ½X�
¶
À
Ye"6Ë£O$èª}$ŒJK]6c¡w  † è /!¸!6" 2„iÎ\	^´òò>÷*?rn¢¨T ˜!! j`½‡vìQD7	2>Ynvä,n_‡§ð#è…†- !o"ö"5 Y oWZ>�ÇÙ¥q‹C	Ì	
§
ƒ­þ½]‚ˆÞ½Ø.ObvNY_v;F‹Íã˜› Q!å!\"}"Û"2 d X|�åÞ}+—äð…	‹	¦	
ª
,2l•ÕÂ ?µ¶ö,6V CÈÎäCƒùÛ5cdj Î ! & A"4l„Ì
û
¡RZ}Äéâ5;++a-�ÐG  * m r rx˜ãòO3­›
%”Zâ‰ªˆBWgmXÀ�twÝ@K]°õ% â "‰"œ"¤"2 ‰ ªC[Ý¦Ä#]c’Pto	5
Z
g
†
×
¦ùMt’øadj¾¨zÐSw K0Âµ'Z¹¼u!" ¹Éƒ·ðh¼&�
µ
X5IÙ¢ö>X‹»%¡
5G4 s *!.!—!ò!9#, % c WäV=ëPƒCŠB	Š	¥	Ë	1 ÁŸá=´a+m*¦Xu,Ú"bF—i !Ú"% ÜÃB‰¬f
Ž
š
W“žõ=`*ºÜWt$+/?J\ÁY¯F!ñ!›"8#8 \ ¯ÂŒˆ±e
˜
™
UV^vòóô®¯°ÂÃ()À>IZ[“”WX«¬­®é./!!!ð!""€"�"Ž"˜"™"š"##7# ] 'è°Ãâ9•î;²‹w»±ÄÁšSgpô•)0<�"±"## ¡­�;V_¯õ_›u°swêU ""‚"#  (ZŒßé]™ÄãïÀ	 œx²ÞÑÂ,›J¤;Œ1jæ�"²"# á Wu>ÁÈ	;^sÕL³T\q ƒ ¤!Ì!Ö!¡"¹"Ð"## ^ € ë :d‡²–'ÜS
]
ò‘SŒÁ€ÅÐÔ&nÊhõQ*\ [!  Œ ç ±<³¼ÀìØ�–=Aq @oµ®(Fåü	WÊ˜'å� Û " j Ìþg”1,0	l	7�<g¸°fi¿t¹	3Ú*tƒ"# ¢Ï¤UÝ•	£
iÒ§ö`‹ f5œG›ëV¼@ ê!"Q" !Ô»xŽá	€
Àîbw`
p1y±vT±u­x “!@"- 
)�êB^šäi4÷$iÒB¡Â×Ç	Á	ì~öÀzÆ&Ÿ¹Ã-KÜÂ F!L!f#5 † [àSy|£©¬¯ÍÖÙÜè÷ý½Jæ¸	Ü	Ùyís¡(ŽX•y³]m­?œÙ<�2Oçî ó" ± bÅð×Ï	y÷	Àû�}�¶Êß¥±6ðø <"³"#$  ÄûHÖq	*	F	š	<
IŒwé²{ßè\eØÎÍÒ€¥kj!x!¬!‘"  â ÿ jvhKVšÖ!M}]âv3Ÿ„ ¥!ã!# XÛÉ	Õ
_t�HUr"%¿º Y!Ô!¢"# ¾ Â/w‹Â†ŠØ=´KMÑ"ÿ" À ?m\<+Tß¹ÅÃêÍ!×!º"À"û"* � Ì Ó NƒÖ�Ú�³ (	r	±	©ÞóPvà’B"�‹Ê§p~ƒ>öR²] › ý!Ö"ë" Ð Þ ì ÷ ø/<;ˆ}€¬ 	Å	P
T
¯Â:üÑ'oN‰à +Y Ó" _ ˜}ÆÝ^
&ðÖ‰”�»ÆËÔCÁiy0ú’	 Þ"##.# e�—Ó	E–T��¢€Õ´ú¥øÓYÇ‡Ê \!œ! 65Úéj�·`°†½cÁ5×Ldní‘—ŸÁ§ !O#/ è ¶¿Z²1Lòôé=…Ä?	i	ëJmÞ›[…PÃ9Q¶ù¹b¡®ÙöŽ>r ß ?!ú!â".  - � 'ö�ÇÒS´¹ý
8
w
Å
¢CæúÉ/v!iKš¤B�©E7´ÇB�ŠÔ!"V"_"W#* “^d~	ç	Ú
:ˆ™�Å?«áƒ§(˜ÒÚ!•?¿èü”l¿!›F P ñ o!2"_#  µ ï "œãw¶õ£¯ß0ŸùGq¤¶1â%E‡GÐÅÏ!­"K# AA]œæ(ßâåú"Ý%BHz¼ˆrºæÀÜ  R^‡Ú+”©~)ëý	ð
%²ož7Cað� "E# Â pÍÞ#
‰
(X'SËƒá«ú™(›:£Úø²   2t-m—î3	
jü¹[ç9±á»Xº·Ûþ+a¡ „"%  h k ~ Íµ¨&;e¹1!m	£	�h@ßÓgÀu·¾Qen‘u## Ê ´%XQÿhõª bÕ¯	N
u
8wµA=˜
ÿL4ì á!ý",# É•f§Ë1	B
ö
°¶þm–Õej¸ «"! £&-^`	–	Ø	à_ÓÎáÅüfa°iygÛ‚`ÍÇÔT!d!…!R" × H¥‰¿*¤
 j$Ç¼œ"½ |ª»`íVs{
'¨g÷6½WA ë!f")  sÐDh›Þ1ŽÍò	j
Å‚ÿ-út´Ñ`TlŒ¡ ��HÑþìªB!�!"E"¼"Â" z 9„ÿÕov	¸�ÁÍV_ó5ŸÌ»U‡|ìþ´!Ù!&". ?¬fü#NŒ†Œ¼’À S�M©W
•
ø
B²ï>¤§pÖx˜ù2zý £ÇygÑ ”!l" 	Û,Â¯â	D
²ËÛGç©ð²Âw	O}@#, D‹"Sê&H­Ë—Êà-yõ5�
æo.c×aÅQíq›0²öv®ôA"M"+ Ž_›åùƒµ÷	'
n
˜Ma”ÕÉÐÅâýr¦­²Ìãî—²…ç.‘â1´fw"? B ’e %Dj�ÓN¢ÃØýÈ	‚	µ	Â	½
É
ó
tÂsŸW®Þ8µ%=¯2¤U;QcÛò:{›'E½Ð!×âÿ!( Cj‹—ÏøC›
	I
M½5§"M¸KN©ºL‘ÝÖ3`�þZ7 « ¾ G!‡!g#; *3­ëôQ¨/[5ÌóX	`í/>©Î¸�ì	]»þJå÷Á9={Ç Äßí$Òj«'É×Ã 2!M!. L mÅ×}ÎI¾ç 	¹	Ý	«Ú:z-{„¢8Á€Íz‘þ´ô^…=A9¦PwŠ! !."{"ô"( ‡ îTºÒáSb¤°Š*Ú.	ˆ	/5X¾à÷3TU�xrkÚòŽD3·"Ø"#<  Î Fz›¡ª­Ë×ÚŸÕä	(	=	|	˜	Ú	å	êõÜé|Ãçît)Œ”ÇY–Öú‡¹NnÌËæ@jÅèï T"2 x È Õ 7P\…Øáq’Üè!*
ÝéøþóK`•t	L
s
î
 ÞärO]5Å®™3x�Ï†Ø_¨ƒ!C#1 ; = ñûV“Øb	d	³	rz|~ÀâøV§õUÁãå+Š~ºËIÙàâð8YÝë�îð7kmü �! ² ¼ò.?º~Ð	è

ü+“/:¦¶(>Qñµ©!="# ? c£²"ÅæFž·25Y×i9çøï�_ ” ù ;!+"´" ÆÁ!žíñ$h�™äàü
e 4–²z�® $!f!í" S £ IøG	©	„ó³DéQÓTY­×éåŒ:Š ­!$ ƒ ù6zL	f	R€�êÊ=L|ƒ]fÙ%€ÎÜ@„†o”ã= þ V!J"2  0 7 � ¨ (;­°þX		+	›	

a
D7JËRxLQxé¡¼àÜÇ[­ªAgúAlà� y!»!õ!2  ¥ÅüŽ×	r³¡Ž	1
=
úoð1¼þ«'9F\j˜îÏŽ³ó�¦®µ6bú¤b — k!Á!’"<# ” š wìLî€ê
i•Ìþëw@Só# ¹›-ö"Ë$·m #   P Ù ã ù k1AW#ÙN)q~‹ v … ¦!  i_¦
×1u^Çã4âc„!ä! $ b ‚Ê	…
Ö
`§<Ž´ôr -!t!£" & ç¬d
uº­Vs#*YÀ(-;ET ï!# à æ Y=&Ç	R
]rKÓÉI×:P‹
p[ ‚ Z!Õ! nËÜû	¾ÉŠŸF&šÙ»?" 	aÃúxA¼I;
Ø‹œõ[×¬ØïNÁ K!«!e# ¿ J.vŠ[Ô
SŒ<G¸¾JL Ò Ý —Õ.;ŒŸ	Ò	¡¦Óù¤‚=±/‘X Ò"Õ"ê" #
#% , ¾40¸ÃÄ
‡I˜¦Ù u>4¾µøKm¨­ûõD“žÀ6
€ E >!á" ´ Á î !›Ì*ô@Þ/}µ±ÜRáàF¢Ï¿ÄÎ! 
 g ³$W§0¢	
µl•dPý  ë ü" ,)]z
^þ,ù#&c!Ž!e"»"Á" >G‹Án,®V
±=ÚUÄïºÆNfÐëØ!%"& A ¬P§‚Ž´ËH
È
_.žáLº1Ë:¨–„#áÑ³2_ýY½ 1!þ!& w Ç Í Ô O„×p‘ÛšÊ)	s	ªßè2{ƒ“ÀNù4†qåw¥iÄ-"¶"×"#  : ¢ú!²	ç
qô **#¹4ŒX9ûd38Ž•÷�^ “ û #!ì"' ‚ ¢  ÷0
`
CQòPw»C ª&8‚i—$P¬­?SX¬f“£a ‰ – œ º!À! “ ™ ø 0ËØýê(pá!# # a ß <�%Æ	Q
¦;J¬½ÈÒOŠó',:oZ � î!! Ñ Ü ù3u‰~·ž	Ã
Ó
HR¥‹ÒÖ<F¬ú’¿	 D =!J!Ô"à"é" í  š=+­´¡	U
°"ÃQàEO M¡eÏê b!$"d"' ¡ Oo¡¦�ù/
G
_
^p-�ñ)O)¹¿73…–#ú”«1Ž’Ãüˆ ¼ # ` ™$Â
Ò
¥Q¤Ê×ßIŠ¼ÇÑÕD;&9
 C í!c"ß"#/   N¥	'(£¸æ)*„•ÌÍÎÏÐ"DøùËñz{|}opŠ‹Œ�Êû%8|!b"Ê"Ë"( ~~½ÇÞ	ë	¸
$(kçm‚ˆ•ª+žEgÌÂòj~éiqÏ-Ë“}!h"s"Ì"/# « ¿¹
—‘ƒ¡,ŸFh¦ùkZ–Ãç)ãLZR”y !"8"% ü �ºó>e•ïXfZ˜j¾	 
}
’
g%F8PdU	Ôk~˜Ç Ë Y"i"Í"+ b’žÈß
[�ÙÍIèŽ ž£û‰–F�ÖµŠõÍjt‚Õ&ˆ.Ì]!~!�!"t") e¾Ô	ì	Ç)ÐölõJnŒ‘«Ñ[¬ûÙL:Èóêj½ÃrÐñÖ Æ"$#0# žÎ˜¾y G§Â"èp’ÜäMS!"P# 6šÀÛêk¸±’ïê^ú6Ø—Ä¿˜9"H# Èº
aôd6“–>î|àâW •²¨ ¶ ¹  !G" H ¬ 7€Þ
‡„™¢-iZlM[eo*ÇÓ[Âz %  »?jàê> 	G"hQvt†æ,tâ:Rú÷¤HÞs„ Ì &!Z"ã"2 é UŠ·Ý[—³f–ðMY[†¿@	j	¾	~
ì9K`�Ge œ\¿²n$lQþu¢ Ÿ·?à ‘!Ê!"Î"% õ ý À4ô2gõçÅ	Gß&V›¢Ä·ÕŸ¯Úà1™È D!h!š!ø!û!¾"ñ"( uF‚ór™3kì^	!
“
h&¢#nÓE
g§ºcl€J^Ëü�8îü° @!j"6   . Ž ÷Ÿ�ÓTâ 

l
P¥ÚD¶çû /6ˆüJ‘ëï0�¤ü×ìòLÊCÎƒ�´8?Ö]‹Õ!ž!u" ( ,9
x
Ø÷ô!Ÿó¸}kÖ‰ÅÍC�V * c�“ÈÉhžµ¼Æ
Ð
\�ÎGJ¶ó�ŠGV‚ö1u€'ª»£gÈ`!¾!	"""W"`"Ä"#  †pºàþ
Ž£HÊé—w"j¶‹›¥‘ê/Fµ‚^!"ï"X#! ÒÃk_Ð;Ç*Ì)ÒŽ£­¸�Úéôý¾|ò•mG Ñ !p!%#( €€ˆ¿�Ï’	Õ	
�šªÑ÷m3öÛ@\ü@ÀÉ˜	ks_ªœ M Q 3"* ”ûFe�		è	ô	,
Û
ÈÆ@¬ZB™ÓKjo’M�°;	ëÄìÀ": !!Ç"& 3 fí	
­
ÿ
‰„žCâ„Œ¨?b"�¬ÆùAJ�–/TÑÞ¬× ò 1#Z#`# ¶ ð x·†3ÏÔOE¿2Fp‘‰öqžÑÆe Ð!"Q# ;SYÏ8õ™ÎH#ðéT" ®"ø"L#  #�äKléö·I	º2µë&zò.H¨ÝòN—±÷‡Ó !!+ ¤°Åà1 úHrŸ¥ 9	x	Ï«è4B¦ã¡�¨ÃÜ0ÕØëˆã'þ“åëo  ¯!Å!# W w›ÈÎµÁÜlç)7cÒ¹»ðâ&¶‹{½î‰êð+ æ ¶! 7vŒ
#
Î‰Id±*˜®sç"5# ¯ ÔðæûÞ“Ô›7£WÅˆÁÝ !:"]#( | BB§L^�Çë9„¢·qàã@
É,²ëC_û…Ù¡©À™µô…»²!©"I# cð¿4x«»
ñ
’Ã³pD[Õ7—?}ã%³© H" _,ÉªìgÕ¯Wr! ž Å &ôbÿŸ8DeJbïVádmÍX|ñ–‘ º !!"q" SˆÛ•*þ	õFX°”½¡°· È!F# U ­ Ã °îŠ
(@TÌ.[{šœb¤îÛ!'#  ß‰×ú†eˆ7Vj„æS}\a)¦/ Œ 6!•")#& I ¥ 8DF$
ì	a|…“âÈõ7Qÿ[f¸ÀÈÔÙœçý*;\‰<~Ûù³ ("9 /;IqÎðÔbm²a�Ò«	ß
=œ)™Y+d*pxš£.„�™¥¯ºèÒî]mNfp¬ÎÑû+~ŽÃI { Ý!+ ƒÑN	ã
Hk­ºzÀì5agw‡Ú²·Úý	4EQY†è!–;Spû+P¸ÉÜ¢ …"ä"* » 
@‘”u§áë.?n¤	4	°
‡#i’¬ÓÛ?UÛþ»¥åÿ�¨ 1 '!ç!"  ) ° !T8Í‡˜[	
!]RoNuâç©mI,JßbtÝS ~ 8!5"8 ¼@Y!u|Ž3kï


©
ú
‚Qý\è:>‡Ý*¼Xm-Nuã+U`^B‚øE€ÌÏš…   Í P!["n"= l q ¸È‚Vq¶ãg—©ñ<NZ2"\‘OsA	n	¤	4
Y
Ÿ¥Lsˆ‘©HiØAf¡à¿³Rvÿv¥ a–¸á –!"% [ ®ÁáÛÂ‡«—
T’�]Š®ñ^Á_'Rfoš=H’rv !"�"—"#   ê Vt‹Þ\˜À¿	ñ:ÀÔ%m¿Ð[£@iå Ë!Ï"& i ?´Î£ý'fºä0k	”	
¢
Ñí aŽho0x¸°4—¬? Ž Ú ’!P"- … AhRp3ö#hö
ÆÖE	Î	xH¿ìrWÉÞd¿lÅž¸°Û5í ÷ E!i!ò"  þ iÁU…™|Þ á!u2žX!â!¿"þ" Ë ö M‚÷�Ù|«	°	O
D!Šœ9³B÷x†Mˆš É ›!ü!Ý"-#< µ&5Y’õŽÙè Kiœèc„üh	
v
9¶Bœ>ù_à‚š¯'„.… h”£ÂPÖc¸a �šÓO ¦ n!ù!^" v\"
'$n&�B‚A»9q¹ï÷± ¬"  ´P
%dôslí2	®	i¯ý´@æÔ8à¶dmK�à!+#   ©%Gˆ¾gš�Ì_	×	ñ	ÿÍû_efkh�_ÌýÆÓ!A!S!D"/ Š«eƒÚûþ%‹Ê–É4¨C
”
÷
±·An�£¦oÌ~—ÖøFæ¨ÿË/{|ýL"k"?#B 2ó.Z–ŸÎ òW	�	ö	&
m
LL`rÍÔ·ÈÏÝŽÄëü!®\ýI£T¥âb±<zDÏæ� 0iÖeÈÕ€Ö†!v">  K 6çí)àÄÖ HžÔ_ã	-	<	{	‡	—	Ù	ä	.4éô9ÛSz
7ÆÕ�ý¸ÄóMËjÊ2„Î…C×^§Ÿ!S"B#' ~-±U’a	áU¦ETâ‰’ìð1g.Øß	XŸhÜµæíyî=jŒe!€!*"2  / 6 � § ¤'ø¯²ý	�	¨	

QÊnƒèï0Éý<KíÆ~²Ûò©´ƒa…Öèù‹9@â< U! Ø ^¥

õ"tŠÆÊ#l  c
„
\q¹‰žÈ9ED Ô:I-HZ:
×—› ôt~¥ø£«3�÷l×½��W d# f )Ý.F|¦ŠÀ+y
´kÙø%cîüÎ¾Ÿ & 9 v «™ÉÊŸöÇ
B1z‚K0©MhøW8•O«ä2¬"v7Ð¤hX¿! ’ *2t¶�	!E«ÿ‘¾nÎa!#"/ }¼ø	ê	.
F
Ñ
]o,œN(¢·¾ål�‡”©ÉÞ2Hƒ!÷ÊCh,ÂÉ$B {!ì!a"g"É") ª =ad‘”îWi½

|
ŒÏHô�Iú‹�9is�Ôæ(¼ÂQÆ !
"X"Å" Ý
Íî˜éx]!Y’=ÆÒÛáµ F"1  tEiqßæ2ÿF!g¡ FuÒDs¾m#k¦tžI]Êûÿž07G¶ÝíƒûC!�!÷!"½"ð")   oœg»ák
Ï
¤×€.5F‡ûI�µêò·UëñÉŽÄºé>f_!½!"Ã"0 2 ‡Ñ�Î‘	ó	

+
¬
þ
�ÆƒB‹ËA>a~ÅI�¢·.Œœ¯S{Ýë^« L Ð !!Y#" …Ä 7w	¹2ÎªÍN¥1ŽÛñ�ê-âõý–°nÿ! d Ò Ä!  { Ku´8ƒ¶p6?
È	+Óšáµc„°V ´ó„* !µ!4#\# ª‘ÂóWfþC¯Ô¼V{¯q!6  .:HïÓCl¯Ö`Ñ<˜*cë`{ƒ�˜¤®¹ç’ÑÇô6PÍÐþe¿a}üˆ}íH 5!Ú!'"”"&#< ( º ÿ‚�Ì“†£Ð	

¯
â
ù
 †¬\«Òny¿Úë4`†Ù=Ü)lü3DM¨ý_ol�DOÈIÜ   R } 4"m") >sÇ�­ÀUãÁ/�Nª“	3
X
–
¡
‘ž¤ðr¨×­ð]¤¯3™–`«hÙ •!O"* Lh�@ŽØg‘o�›T{	C›qø�™®È-{“²¾AÁö�·t�ÒN ™ ö m!/ ¨‰$d³Ùý$O$c‡½­	Ö	ð	ü³?¥å÷ú7^d%œA@þÊ.
ÅÒzöß!C">#; 5 1ò•ì(ÍÃÕüG°‘€	ã	õ	èóTqÉ‚ÇÜ�	­È;[
HÔS-ž±ñ¨³ì/x„Õíø
¦; A# ÓÜ-ƒ
	³[špss¤ˆ�8~c#% 
÷	é	-
E
A‹+›0  ä/HLk€�“È1NªhrÓ�PÁ#!È"  nf�	Î
Ü
ý
Ìf AE4†�Cr=èêð<
ÃÅàK !$ -GîJt®©µ6ò©MbýBz®‚—0ðƒUd³zƒ¯®Ã!3#[#8 =Kg§þ�×#T�šËâ.zï	
 
®
á
 £…q¾ä€…ØÇc$ïûCL’§±½õœ®-2ÛõØ õ B"N"/ 4 Òë'ÌÔö‚
@Šçòp/eŸD3…Ç:Ž.<R,rŽÇ0r‡œƒÔß÷b#8 EFÐÑŒÖI˜™¨áî	Ÿ
?ðñdƒ„Æã9�-Æ;@A#­îúBK†›¼1€�‚ÒÓwxy­Úó ô 2#a#  5  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        (                                                                                                                                                                                                                                                                                                               4                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           	            
                                                                                                            (                            (                                                                                         /                                                                                   '                                                                                   &         !             
   
                     :   6                                                   
            $                           	         	   	            6   $      2   (      3   *   "   -   ²   o      %          ;      …                     (                                                                                                                                             4                                                                                                                                                                                                                                                                                 	    
                                      (          (                               /                             '                             &   !     
 
       : 6                 
    $         	   	 	    6 $  2 (  3 * " - ² o  %    ;  …   �÷  �Ÿ  �Û  �5  –  �  ¥k6š­	_	®	¯­	ï	ð	Ü
A‘Ì+³‚�­ÇÈÎå`€*HbfÇ,r¹Ôô–DVü©eƒÊ�¦T<) [	
Ï`�Ï"¬Î²!‰*A†,¦í  @"–{ã†ß\ß\<¡D	ë ^Ü
AÌ‚�� ,¦ÆíJb¾ú3<ƒ;	D	M	†	
Žpë´d áù68b}›^‘àæÞ2IÔÚOs¢­.Z®ñcœ  ¥Ü[\4‰O*¿A™©ó¿’™·©¿™©™¿¿™JAeƒÊKöš�x¶¦ž¦ E!¬"¬¿4†üDOuäx¶=/"1óVç[©]4HŠ1ÆóÑO8AN#YVgZ#[©] í·F#G	yÃÕ‘ 	EE¹Jð2œ
‰€6nù€	qÎlÑJGL!»U"Ø�Ï	¬ô�Ï	¬Ó
‹FQ†Ù“D#ö·¾G#Ìø„QŠ ð²…¯'Œ!©Œš?¯Zäì4zë{Pþ6	†ïõ"� "°5/©]«è�=Äß“2!] c•Û\
R‹Ägíà‡	.jC=X"nQ†Ù“šìªùÆ!D#DÂ Õw4¸× ï
Æã¬Ô»Znô!I"i#nI"i#Mî0€úÞ ;Å	P
ÑN‰Y –]�Ê€kú³nI"i#©]=Ðã(
Ô€ÃúïÂV»=áF"šìb~øÏòD	Ýc~½kù"=	éYN_ÓÎ°þË
§ÛI<K.=üŸ `Dšnvì¶(ÅQ6
U#©Œ¦)
�
Ê
šî?¯Xu€�ÂÖ7‰3ÐúZÃÏäÿGì4RyzØ Ô "# �	r Î
 ‚¤Ftž¶"!P½Pñ*±n@g…´Ì6	õ	á
Ìp‘¨Çy¾Î…ØÙ/MRûC¾á0��/Ô…¸Ì•Ñ Ïäš ÿv	¸¤,áüþzxF8(°Aè�L%
KÃáåd5yK	øÉè�Ý…!²!F.@g	¾�¬ �Æã(
¬ÊÔ€Ãk»úïÂ³ZVnô!I"i#.
“õVñ"Õw4·@g	¨/¾�g»FºÃ"JV ,íY]â3w~ñõD	V	Ì�ºê<(ÙÝky¤Pc�;X^z~§«áüc€íCh• @g	‘¾�ÕD	Í	w¾Ýc~4*\ÅÐQ6
Ø
 ä©ÐI˜Ž²½"0"U#® Ó¢5…c77!%�_S!M0&9í!9WÍîÒk_Ð;)Ž£­¸|G ãÔ×íà �	‡	.rj C=Î
 X"ŽNÌš¯ý	Êè@cRéeÁÆ _Œ¾G#ŸÆ Ÿñ€«ÑRü‘
ò
fsÁ½_Œ<ö”?P·¾nSI"G#i#¬ YÈõF[K�ªÑvÌVÓôüQþ:C–ÎúTuºp£g· ñ"P#Z# ñÂ ’	Ç*òª@ 9¤áüïU‚ˆ}'"³5à´) Íî<KïU‚¾G#F‚îtž¤¶"Ð"6y ž³!É~ÖL"yÜ!Ž—çw" ±"U.'

ü“Œ![	N©LG!ôÐ äÐIa˜Ž²"M#]}	æ	Ù
Äª—Ñçk¾ ð 1"^#1ò•‚ÍÐ€	õ	â
q¬ÇÜ�y­¿ë4[`†Ù
HSü3Do/OÈÕ…Gî"P½P.ÂUñ¦ Xt{®±	Œ	§	¨
�PmîWÅ*T}Ú]A„ÕçŠËÎ™áO!· +ì¦ù�D	�
î•Š¨€�×17KoúûñØ' 8 !…62Î©MN01ð�õ¯d Ã!Dk¸ÐQ&	J
q
ì
¼ˆ!y"‚3“
¢nE§üj"ù�D	¨1ñ8 !Xt{¨
�PW*T]AËÎ™O!¦ ®±	Œ	§	mîÅ}Ú„ÕçŠá‘�››ø�™®-“Á·ÒN m!Ñ
 ÁÖ	ë}y%£¯ß0ŸùGq¤â‡eÓ ¦ BXt{®±	8	Œ	§	¨
�P—mîçê3AWæ‘ÅÐ*/T}Ô×ÚÆ&O]ÏA„Õçê`ûŠËÎ™á4!O!®!“"1ò…•‚Í6Ð€	õ	â
2q¬ÇÜ�y­¿Îë4[`†©Ù
HMNSü3D01ð�oõ/OÈÕ¯d Ã!ØçJƒûg	
^ƒ„gOb`Œ¥ ]" GÕ	)	™	vè±zçÍÌ¤w!-_ÓÎüa°yÇÔ…!( ÿÌ†
 \n¨lIÜR } 4"Ô	¶	w&°y´æ[$=’ c9œ>à‚'”šO n! ißÿF!g usGÝƒK ÄÖH	9z7�ýó„Ÿ!y¸ÜvŠ÷ø@€"œãõ¶—`	à�Áá1Váf%gÛG»U+ad! K ’ ÄÖHißÿ	„
F9!g z7us�ýó„GÝƒŸ!8‡˜RouâßtÝ8!%_	×	Íûeh�_ÌÆÓS!Ýˆ
ªùšÙEÇ!-Gî®bz‚—dÓDoˆ�‹ rCœ0i
Ä�³•ñs³Ðñ=SÒXŒÆë�Ð§©§�¢àãC_…”©½™°· F#I# Ÿ�Óàê>â 	ÇÚ"*h6˜�üÒòGLÊ"ƒè¤òSÖ]!&!ž!‹ H�hrÓP!wâ$Yá‡VlWs$ ˆ"mÊ 	³ò”ë]	ú	K
r
í
Ýã"qP\f˜N™ØLd¯ é ‚!>"ˆ B¥b~ø÷c½yÏJ&»c�“h¼ÎGó�Š€'»	"W"Ä"³ññ=§rC0i
Ä�s³ÐSŒÐ© 	ò”ë]	K
r
í
Ýã"q\f˜¯ ‚!‹ œ•ÒXÆë� B8	—çê3Aæ‘Ð/Ô×Æ&OÏê`û4!®!“"%8‡˜_	×	ÍRoûuâeh�_ÌÆÓßtÝ8!S!† S£¯ŽÙ�2ü óf	FUÔ˜Ç ­"K#p!U ­ Ã °îŠ
(@TÌ.[{šœb¤îÛ!'# .:HïÓCl¯Ö`Ñ<˜*cë`{ƒ�˜¤®¹ç’ÑÇô6PÍÐþe¿a}üˆ}íH 5!Ú!'"”"&#Jkè:RXÎ8	H	ô´Óçê3AD/oÔ×ï&ˆ�§êñö†!®!÷"	 ³ ²Ë#Vó/(
]°ýÛ”�!d X|�åÞ}+—äð…	‹	¦	
ª
,2l•ÕÂ ?µ¶ö,6V CÈÎäCƒùÛ5cdj Î !T ¤ aˆ±íEùª	›…d)6?Uowåí-R\z|`Z›·Øæ)�;¥. ‹ Ü!(#Ø ^¥

õ"tŠÆÊ#l ]ñÆóÑR>	}	æ	7
Ù
¡êÄlåªÝÈZ—ÑJOb™ç8A�k³¾Æ ‰Þ ð ! "1"U"N#V#^#¸ +ì±6¦�
î0–ª€‹�Ø7^¬Kpéû¢ßå�>Åà”BM_Ø( 3!…Ä 7w	¹2ÎªÍN¥1ŽÛñ�ê-âõý–°nÿ! d Ò Ä!… AhRp3ö#hö
ÆÖE	Î	xH¿ìrWÉÞd¿lÅž¸°Û5í ÷ E!i!ò"& d t ¸ +4ì0X\|�±åAzÞ"6}¦+—4l„äðô:	U	y	…	‹	¦	
)
�
ª
Ì
û
,2Jm¡Õî0R–ªZl€•Õ}‹ÂíÿD�ÄØé
7^‰ ¬¼â5;?Kpéû+ðø§µÃ¢¶ÿGÂßåö,6�¡©ÄÝ1:>V|ÈÙì+WarÅàä	 0äÿCÈÎä-Cƒ”æùBM_yÛ5ch�ÐGdÌØ   ( j Ä Î !3! … ¿ þ 	aiÁÃúAJhRp.3ö#hvxŠöAU¼
I[ÆÖE	Î	;
Ô
x…ØH‹¿ìr™SWœÉÞõŒ[d|¿×<l¬ÅÞž¸ ØG°¸Ûá!5u¾2ïJLNžÁ í ÷ E!K!X!i!«!â!¿"ò"þ"e#R^‡Ú+”©~)ëý	ð
%²ož7Cað� "E#ƒ ù6zL	f	R€�êÊ=L|ƒ]fÙ%€ÎÜ@„†o”ã= þ V!J"j#^ € ë :d‡²–'ÜS
]
ò‘SŒÁ€ÅÐÔ&nÊhõQ*\ [!nf�	Î
Ü
ý
Ìf AE4†�Cr=èêð<
ÃÅàK !D üxÉÏ¶Âõm7=FIU[8;J_zè!	S	º	(Óu{º°”£9~/Îø{ÆÿÝã_Ÿ'Œ¤§ëxÉ# a ß <�%Æ	Q
¦;J¬½ÈÒOŠó',:oZ � î!E d R^‡Ú	+”ýX|�©Ûå,~·Þ)P}Â+>—ë<K¯ä{éð…	‹	¦	»	â	ý	
D
ª
ä
ë
ð
,2•²Ë[®»ãlŠ•«ÕÛ%¤²Â:h0o ?GY–žµç7C¶5ö,6‡Va©ð ²ÞCÂÈÎäCwƒù¨Û	5ATcìOdð}j � Î !"@#E#6šÀÛêk¸±’ïê^ú6Ø—Ä¿˜9"H#  &ï     @          7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C$� S0@HZÂ|NÔèá›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  6  8  8  T  b  u  z  �  �  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1668" maxFusedRunOn="1668"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1668" maxFusedRunOn="1668"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="14" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1668" maxFusedRunOn="1668"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="13" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="12" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5928" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5137-5928" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5929-5997" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2841-3635" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4381-4966" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="9" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2003-2431" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2445-2774" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1733-1957" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2625-2760" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3809-3930" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5998-6102" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1668" maxFusedRunOn="1668"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>5a32cdf3-8037-44aa-9f16-5433eb8f8b89</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="11:50:29">2023.6.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..6165" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6165" colors="black"/></BottomStrand></StrandColors>  (     8          7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C$� S0@HZÂ|NÔèÆoMÒ<ìK‘ò¥8m>Çˆúä£ÓÛŠ*?ïü�¯¡þ÷¿x¾¯–U„£lÚ.B-®k€ €45 hdÐØà0€î`ë„0T_N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬VSý~=·«÷Ï>¹…B€ë²«ÀsÃ�†ûÓ0íŽ®³÷ýz*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹‰Ÿ,~ääNƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË¿þ`  c  6  8  8  T  b  u  z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1788" maxFusedRunOn="1788"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5997-6101" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3808-3929" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2624-2759" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1732-1956" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2444-2773" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2002-2430" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4380-4965" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2840-3634" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5927" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5136-5927" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5928-5996" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1788" maxFusedRunOn="1788"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1788" maxFusedRunOn="1788"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1788" maxFusedRunOn="1788"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:32:46">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2371" colors="black"/><ColorRange range="2372..2373" colors="magenta"/><ColorRange range="2374..6164" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2371" colors="black"/><ColorRange range="2372..2373" colors="magenta"/><ColorRange range="2374..6164" colors="black"/></BottomStrand></StrandColors>  (     =  *        *7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C$� S0@HZÂ|NÔèÞKÒ ˆÑ›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  6  8  8  T  b  u  z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6018-6122" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2645-2780" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1753-1977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2465-2794" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2023-2451" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4401-4986" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2861-3655" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5948" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5157-5948" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5949-6017" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:32:29">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></BottomStrand></StrandColors>  'ü     3  $        $7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C$� S0@HZÂ|NÁ½ä½"�½7H0ó±.DÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚  c  6  8  8  T  b  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6012-6116" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3823-3944" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2639-2774" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1747-1971" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2459-2788" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2017-2445" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4395-4980" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2855-3649" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5942" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5151-5942" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5943-6011" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:32:9">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2386" colors="black"/><ColorRange range="2387..2388" colors="magenta"/><ColorRange range="2389..6179" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2386" colors="black"/><ColorRange range="2387..2388" colors="magenta"/><ColorRange range="2389..6179" colors="black"/></BottomStrand></StrandColors>  'ý   
  3  $        $7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C$� S0@IN‚|NÁ½ä½"�½7H0ó±.DÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚  c  6  8  8  T  b  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6012-6116" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3823-3944" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2639-2774" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1747-1971" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2459-2788" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2017-2445" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4395-4980" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2855-3649" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5942" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5151-5942" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5943-6011" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1803" maxFusedRunOn="1803"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:31:53">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2386" colors="black"/><ColorRange range="2387..2388" colors="magenta"/><ColorRange range="2389..6179" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2386" colors="black"/><ColorRange range="2387..2388" colors="magenta"/><ColorRange range="2389..6179" colors="black"/></BottomStrand></StrandColors>  'ÿ   	  5  *        *7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C$� S0@IN‚|NÁ½ä½"¸Ñ›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  6  8  8  T  b  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6018-6122" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2645-2780" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1753-1977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2465-2794" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2023-2451" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4401-4986" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2861-3655" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5948" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5157-5948" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5949-6017" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:31:40">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></BottomStrand></StrandColors>  'ÿ     5  *        *7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@C� S0@IN‚|NÁ½ä½"¸Ñ›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  6  8  8  T  b  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="246.22" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1621-1634" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6018-6122" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2645-2780" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1753-1977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2465-2794" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2023-2451" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4401-4986" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2861-3655" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5948" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5157-5948" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5949-6017" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:29:34">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></BottomStrand></StrandColors>  '÷     -  *        *7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@Ã� S0@IN‚|NÁ½ä½"¸Ñ›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  6  T  b  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ë<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" translationMW="246.22" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1621-1634" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6018-6122" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2645-2780" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1753-1977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2465-2794" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2023-2451" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4401-4986" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2861-3655" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5948" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5157-5948" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5949-6017" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:29:26">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></BottomStrand></StrandColors>  'Í     1  <        <7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�KÁí±0@H@0C”è'ÄìÞKÒ �k�½7H0ó±.DÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚  c  H  f  t  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ½<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Linker" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1827" maxFusedRunOn="1827"><Segment range="1639-1652" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6036-6140" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3847-3968" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2663-2798" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1771-1995" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2483-2812" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2041-2469" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4419-5004" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2879-3673" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5966" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5175-5966" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5967-6035" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1827" maxFusedRunOn="1827"><Segment range="1624-1638" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1827" maxFusedRunOn="1827"><Segment range="1609-1623" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="1827" maxFusedRunOn="1827"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>8e23bba4-fd6d-46ce-bb79-d8e75ccdc0ee</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="6:29:14">2022.8.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1653..2410" colors="black"/><ColorRange range="2411..2412" colors="magenta"/><ColorRange range="2413..6203" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1653..2410" colors="black"/><ColorRange range="2411..2412" colors="magenta"/><ColorRange range="2413..6203" colors="black"/></BottomStrand></StrandColors>  $     -  *        *7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@Ã� S0@IN‚|NÁ½ä½"¸Ñ›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  6  T  b  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5948" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5157-5948" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5949-6017" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2861-3655" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4401-4986" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2023-2451" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2465-2794" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1753-1977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2645-2780" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6018-6122" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="874-1590" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="3" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1809" maxFusedRunOn="1809"><Segment range="874-1590" color="#00ff00" type="standard" translated="1"/><Q name="note"><V text="EGFP;mammalian codon-optimized"/></Q><Q name="product"><V text="enhanced GFP"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="Linker" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1606-1620" color="#ccffcc" type="standard"/></Feature><Feature recentID="1" name="Linker" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1591-1605" color="#ccffcc" type="standard"/></Feature><Feature recentID="0" name="Linker" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1621-1634" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>f14088ec-eadf-40e9-b21d-70927be69aec</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="3:36:43">2020.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1635..2392" colors="black"/><ColorRange range="2393..2394" colors="magenta"/><ColorRange range="2395..6185" colors="black"/></BottomStrand></StrandColors>  #>     !          7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�@Ã� SS Ÿ°oy/H‚®4fôÝ ÃÎÄ¹?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æ  c  6  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="Linker" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1591-1605" color="#ccffcc" type="standard"/></Feature><Feature recentID="0" name="Linker" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1606-1620" color="#ccffcc" type="standard"/></Feature><Feature recentID="14" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1794" maxFusedRunOn="1794"><Segment range="874-1590" color="#00ff00" type="standard" translated="1"/><Q name="note"><V text="EGFP;mammalian codon-optimized"/></Q><Q name="product"><V text="enhanced GFP"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="874-1590" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6003-6107" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3814-3935" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2630-2765" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1738-1962" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2450-2779" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2008-2436" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4386-4971" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2846-3640" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5933" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5142-5933" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5934-6002" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>c84247ce-c15a-47e7-ae36-0b174ccf4021</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="3:34:25">2020.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1620..2377" colors="black"/><ColorRange range="2378..2379" colors="magenta"/><ColorRange range="2380..6170" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1620..2377" colors="black"/><ColorRange range="2378..2379" colors="magenta"/><ColorRange range="2380..6170" colors="black"/></BottomStrand></StrandColors>  !«               7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�B¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄ¹?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æ  c  5  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ²<?xml version="1.0"?><Features recycledIDs="0" nextValidID="15"><Feature recentID="14" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1596" maxFusedRunOn="1596"><Segment range="874-1590" color="#00ff00" type="standard" translated="1"/><Q name="note"><V text="EGFP;mammalian codon-optimized"/></Q><Q name="product"><V text="enhanced GFP"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="874-1590" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5983-6087" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3794-3915" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2745" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1718-1942" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2430-2759" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1988-2416" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4366-4951" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2826-3620" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5913" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5122-5913" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5914-5982" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>c84247ce-c15a-47e7-ae36-0b174ccf4021</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="3:33:7">2020.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1590..2357" colors="black"/><ColorRange range="2358..2359" colors="magenta"/><ColorRange range="2360..6150" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1590..2357" colors="black"/><ColorRange range="2358..2359" colors="magenta"/><ColorRange range="2360..6150" colors="black"/></BottomStrand></StrandColors>  !š       
        
7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL÷Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IJ•éN‚|NÁ½ä½"¸Ñ›Ótƒ;ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  c  8  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ÿ<?xml version="1.0"?><Features recycledIDs="0" nextValidID="15"><Feature recentID="14" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="874-1593" color="#00ff00" type="standard" translated="1"/><Q name="note"><V text="EGFP;mammalian codon-optimized"/></Q><Q name="product"><V text="enhanced GFP"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="874-1593" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5986-6090" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3797-3918" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2613-2748" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1721-1945" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2433-2762" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1991-2419" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4369-4954" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2829-3623" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5916" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5125-5916" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5917-5985" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>c84247ce-c15a-47e7-ae36-0b174ccf4021</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="3:28:53">2020.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1593..2360" colors="black"/><ColorRange range="2361..2362" colors="magenta"/><ColorRange range="2363..6153" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1593..2360" colors="black"/><ColorRange range="2361..2362" colors="magenta"/><ColorRange range="2363..6153" colors="black"/></BottomStrand></StrandColors>  !˜               7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô”©^”è'ÄìÞKÒ �k�½7H0ó±.DÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚  c  2  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ÿ<?xml version="1.0"?><Features recycledIDs="0" nextValidID="15"><Feature recentID="14" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="868-1587" color="#00ff00" type="standard" translated="1"/><Q name="note"><V text="EGFP;mammalian codon-optimized"/></Q><Q name="product"><V text="enhanced GFP"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="868-1587" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5980-6084" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3791-3912" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2607-2742" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1715-1939" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2427-2756" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1985-2413" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4363-4948" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2823-3617" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5910" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5119-5910" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5911-5979" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>c84247ce-c15a-47e7-ae36-0b174ccf4021</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="3:26:33">2020.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Å<StrandColors><TopStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1587..2354" colors="black"/><ColorRange range="2355..2356" colors="magenta"/><ColorRange range="2357..6147" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..866" colors="black"/><ColorRange range="1587..2354" colors="black"/><ColorRange range="2355..2356" colors="magenta"/><ColorRange range="2357..6147" colors="black"/></BottomStrand></StrandColors>  .      _  4         47»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±.DÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  M<?xml version="1.0"?><Features nextValidID="13"><Feature recentID="12" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5260-5364" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3071-3192" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1887-2022" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="995-1219" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1707-2036" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1265-1693" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3643-4228" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="G418" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2103-2897" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5190" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4399-5190" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5191-5259" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="862-969" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>c84247ce-c15a-47e7-ae36-0b174ccf4021</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="3:26:17">2020.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  i<StrandColors><TopStrand><ColorRange range="0..1634" colors="black"/><ColorRange range="1635..1636" colors="magenta"/><ColorRange range="1637..5427" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1634" colors="black"/><ColorRange range="1635..1636" colors="magenta"/><ColorRange range="1637..5427" colors="black"/></BottomStrand></StrandColors>  ý7zXZ  æÖ´F !   t/å£á†Í] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x‡ç3ýeˆ\÷¼,™0”6»*NÈØã…‡xrøým©à!>E¥_v�«ÃÄ`“I?¢¥|X¦ü.l{ÄÈ€gÆfB®äÏjHõ—kâª5ZÅ*,€? [.Úqy#QÔ#]P§M*0,Ô[_e„6v›åwæ=ªjÓS�Kj)f	ZèÛ4ÍYnÝ²—zä}–~Ý	|¿ùùúrÑ àU/uŽ¶ô<RçC™Âä+¸ŸñÈw‚{œÐÕlµ~‡’±ÆÔ’�ß¹Õ®‰vËˆnsƒº+~µváw[˜‹¬·ËG¯¨p³ÁÔ,)é”’j|ùœîU O¢Ó»ºWƒ¸Cä=^5Ã«°Ê¦CSþ6–E™ºÆÁ…šLÂX§Yä>º^Š$�ùå^v’ÞÉ¨åJà¼��Å�oð†ÔšGw¶�¸xš¥e¶í¯{òëµÊgcDæt
ÿ@0S$hMVÇRßÖÛ¢§“×>»Lk(ÏìŠë"G‚Ñ|�,åÞùýôðÜ–Å�×½Îò‡J¥C—!³Ÿ0”²$™£¿à–;$³dåÃön:c0ýg–ºí0{Bb¾IÔa»¡ø´'tuPº>6…ôÄü£\{käŒŠ>îx|ßò81Hxšzdð£K¬  —Ï×uü^®i6À0-ùÂUÈäw4UbR¹Eå¹ß[êTÌTÃš¼3‘Æˆú!Á6E6 ÑužicÌ}}ƒRÀœdÃÍ�kÐ%èH»É�“ÏA•«†òs†‘w[¹o>¢ñ3›Qâk!å{Rù^ôl1{Èµ @´Ç)@¹-Ç<oG�ú9ÇnàUìïC'WaNyÒb_RØŒó?ØU}¥$Hðb‡~‡¿A 0›ê/è©oXÿN­g¾å9€Çßð9�ÿÓ&m²�\ëNÔr++h–o€$Ël$QùúZÄU�cCã�îOüàÅ¯ÝÙ‚ŽÂ®C
}vó¸�›÷ñ¿£Xf¬Î
ž+¬WË¡”‡qäô©œÖ”%ÙúA¹"As+JQLIàùæü}L"Å-FëOwŽéÝ7Ö·ßz<À�"]á­|¬šK�eâv(­NK—­€^þ/³zØ¦Nz²ÖÈnËœL<˜³^ØB ðL$ˆ‰7™Àµôw¥eú7oôI/œ™¶<P^ ¥ò²´Ê yÔß¨ßtG!½Ç+Æ¡á>Ö¡²ßy
«!à�níWêø;FÛ”·…ï¯¤„ÑÀÈi�údå-„vFëÅ‘sÓÿªN#›îÄˆ°bCê'.“kQ÷(1ÝÖ„&lÿÖ(�ZXÉ2Äñëk4øš¿‚ÿ/Þ‰ºÏšƒ%^ž\ÒJfôo×X¯Æ‡¥´ívºßÉ	ÆÒ½kWd¨ú@fïVÊwÔóàAƒÝ¸Û;HÈÖv-;Ê½1g-¾ò�G!ÒXbR†CµãàKlÔnàœš¸kõˆº|ÊwCi
Wú<’‘ÃLzš:(f eølØÀ×’£T*ñÃá�€æD²±1Í9ö�ûC}ÚøŒ€Üº8�¤t+Ý§ððXqÖìw¡fýÒ¦ŸÕÛµã>ë o¾`$Ä©^XppÝ „á´ž�K'’ªWù
òÀýæ¸S‡b�¥ÚîI]¹‘“Å°¢ËË¢-µð¿c¼çH´ÑƒÉ{œió/+“U*÷ /N˜Ÿ»Œ´Ç-»Æuú§ƒ°«;lB8/«4Ö¡®Á—å–MËH@wv_ßs¤‚+dt5c$aõüÞ¯óœdªFç»êµGÊß’X]Ùµ¹öj¥,È­o^k£ÌK½t>W¨ÁÆœãQóÜxËhCÁT|Æ)�*›ôÄ·º”¾ÞRþ“è¸ÐŒJ}]4\ðt§Ù™¸ÞÊöÜˆ¥òb÷Šj¨ÅÔ®êi'˜{ÄKUT
»î=ºÓÿÆè`a×k†nB.óÊœË×ªˆü�”Âøj²ü™»³t"èù†�~µ’´cR¯gé_pµI´d³…�±—úéB7“·_ãP;,¬¡MósŠ¿�Û—æ {aÈ_éj„‚$¤ ßÑ$O)!—ÙGX4:ãÃ …U#WnZ'œ¾d�Nýõ;õaÇ P¤¬êÆ7V/âIúÞ
;ƒw[Óý•.-©T‹ÕU‡ùPùÑN®0›Þ};8|þ‰:cØ¢”	œ5IÑ£F\1èÉ�DÊª*4‚^CÓµáë¶vžp«EdUPP'þˆ*ç×®»é±Ac­õ·'8§–Öþ—sô4wž7·�B/—ÎÇ8¡àe’g]Áq+	÷ŸäÆ>*þTv§m�z=ˆvçÀ¹j*‡Þšå�|qã#lÔ¶ˆúJÑ=ÙÕ›t##A{AÊ¢®ˆa1¨.o|dÜ@X�cÕ‹YùöæØÞZK|k¸Úå¬8:^o£XZê�æˆ¬ÅIß+Ê¢}ã/·5<¦Œ¼þ@·Ò�˜¯·)ÊA3ñFÅ…Û©®‘€ôé4Tà–ôÿ@|½¾äpãòÿ¥Ž¦<‚ôc­¿¥÷ rF¹|ñÚà¸w•œ¿¼ÈÜiË`	¹¸:ûêÃÖÎ,•WT®ynÁg|wB"U‡�”	@‹FÜqj^­Û1ì�ÉÓk‹,´dIš(Cˆµ©hÈ½à$)sï#çš0Ú�£XåA*0˜Ú…A^|GâÄ]=¼#ó9<Ò tÛ8ûKn3õà9��ª~º³J+¤oç zäÍê6Ú‚[ -ã6$@b|Â®l?¦#Ù§M—C_~lÒ
jœ¬x(¨Bž'!>È,‘Í‚ŒlýTíH!¨û¶„ž0p†¼ßšê®õ6¿‘Î]LB0R¥¸c)Y¦-+£à0“�Ðê1ã9/¾ Aƒ-WÂƒœR{gùÓ¤Ðìò‰U¶gøÐçç¶3nGÑÍÇóÕvÐÐ  ª— uƒÉ÷µ™c¾”Í¦qr3šwy'Ø�Ýpï’E‚ºœþM€u¢kÔÏVé¯oZþkë¹(|IT¿-^wrœû©±‚‘ë¹âzc¬¡+ÿú8…]V¾F�]AIÃ‡ÝÈ—1�A›{–\uÔôÿÍ	„E‰ŒÑeÔöMb}ý¹ý—bè½òaPÔ¥f«‘>cÿV‚§(eÖæZÜIwSøÙ—À8éJ’Û‡õ,~ÈÃª23›�7x¯Õ�ì!»¶³sáÞ�™Æ¦?géü!,Í¤"‹ž!ú©Í§©ýS C­ï&°Ì<qß˜˜ ËéÛýìëkLÐ›p¼ûVv3ç<³0£Ê4‰ˆú0C| }:E·Ö]„(ÏVå(çEšóÆPÜ³¨'¡~;uAUv£äRm"þPÏâ�ÔWþ‹{iWQ-áY<‡Ä.(/ØQòcácõT�â‡æ @öÑÆq•0s ‡Gfò ­GY´Âº�ÛæSÁv´<dëÕ}úÛbü;IÃÐjè¢ýscp…‰ÊU^kãÆð”ßÕîas6b™ã<µþMÏ½ÜµŽ/çS»`µ„Ú+”«ÞÊdâ¦âfoˆ¨îz¢6©ÔÜåAwžØòÿ™•c”µZ^Cn÷hÃ©X€í‹Ð½ÿ‚„Ã¤ô\ã.ê‘²é³DÛ%�üû 
F	Å3µæmiYl_�ýçóá³F^p¼î•ž¹Ð”%e–`3P^‰í3çÔmƒZÓK�þgË]=èÞ57%�T÷®¯Pn2Øº<ÂbQ:å·Éhîb—ÛåbÔO°›@­ÙÌiõL‚ª„æÇXø—bÃ§Ðïƒ…¡íñÂ†FˆÆ}Öê8+q”LáÃpoŠœûž¦t[¿c42gø=¦åÇ©(#ýõÓ¨D.Í†ás:>ùPKŠgM^û§L
¥1M�ð×Æ„ƒ°�ƒ8Z~ÉŒG±¢ú}ÍM÷­ Iˆ®çãTL<ë½? eœTÛ&N€È‹ëfÙŒ‚‚–‰Û‚2Ó¢Á±(.Þ	kÄ-pÞ�¥øé}c =mý 0­äý¸u®]ü—Q^¥€>”}8}ºvX—[Øõ£ì3“Wü ÿáÎ)"ß¤iKa”Qm¼Én¸×1ïa�ôÙjõ@Zs»òC•Õº²sp˜q!Ðìù“LŸ8¢åáþ‹
 h*?¿ã„uªq ¾¶C%#4p�ˆ‡Ãî;ÔµE~
K¥sð¯fgkx)ècV-îˆ¿l¼È³‡¦      ¨Gœ÷Z"Q é€� 
ò Í±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4560" maxFusedRunOn="4560"><Segment range="1621-1635" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GGGGS"/></Q></Feature><Feature recentID="1" name="T7" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8899-9003" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6710-6831" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5526-5661" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4634-4858" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5346-5675" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4904-5332" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7282-7867" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="G418" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5742-6536" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="12" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8829" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8038-8829" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8830-8898" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="13" name="Kozak" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-877" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="14" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4560" maxFusedRunOn="4560"><Segment range="1606-1620" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GGGGS"/></Q></Feature><Feature recentID="15" name="Linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4560" maxFusedRunOn="4560"><Segment range="1591-1605" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GGGGS"/></Q></Feature><Feature recentID="16" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4560" maxFusedRunOn="4560"><Segment range="874-876" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="877-879" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="880-1590" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"/></Q></Feature><Feature recentID="17" name="EFTUD2" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1642-4560" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  §<Notes>
<UUID>662857d4-6941-4166-89a3-89a845852a39</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-EGFP-EFTUD2(human)-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="7:53:3">2018.3.15</Created>
<LastModified UTC="22:25:52">2025.9.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             +<StrandColors><TopStrand><ColorRange range="0..1639" colors="black"/><ColorRange range="1640..1681" colors="green"/><ColorRange range="1682..4558" colors="black"/><ColorRange range="4559..4559" colors="light blue"/><ColorRange range="4560..9066" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1639" colors="black"/><ColorRange range="1640..1681" colors="green"/><ColorRange range="1682..4558" colors="black"/><ColorRange range="4559..4559" colors="light blue"/><ColorRange range="4560..9066" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
